反义X区RNA和反义P区RNA小鼠体内抗HBV的比较
发布时间：2020-11-04 来源：文档文库
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文档收集于互联网，已重新整理排版.word版本可编辑,有帮助欢迎下载支持. 反义X区RNA和反义P区RNA小鼠体内抗HBV的比较
【关键词】 基因疗法；肝炎病毒,乙型；RNA,反义；小鼠,转基因 【Abstract】 AIM: To investigate the effect of antisense RNA of hepatitis B virus (HBV in X region and P region on replication and expression of HBV gene in HBV transgenic mice. METHODS: Recombinant vector plasmids expressing antisense RNA complementary to HBV X region and antisense RNA complementary to HBV P region were constructed and injected into transgenic mice through tail vein. The experimental mice were divided into blank, PLXSN, PLXSNasX and PLXSNasP groups. HBV DNA in serum was tested by fluorescent quantitative PCR and NestedPCR. RESULTS: The expression of HBV DNA in serum was inhibited in PLXSNasX group at day 2 after injection and still inhibited at week 8, while it was inhibited in PLXSNasP group at week 4 and reached the lowest at week 8. In PLXSNasX group and PLXSNasP group, the strongest inhibitory effect on the expression of HBV DNA reached 58% at the 2nd week and 8th week after treatment respectively. The serum HBV DNA of 2/8 mice in the PLXSNasX group and 1/8 mice in the PLXSNasP group became negative.CONCLUSION: The antiviral effect of antisense RNA in X region of HBV was earlier than that of antisense RNA in P region of HBV. But the difference of 1文档来源为:从网络收集整理.word版本可编辑.
文档收集于互联网，已重新整理排版.word版本可编辑,有帮助欢迎下载支持. antiviral effect between the 2 regions was not significant. 【Keywords】 gene therapy; hepatitis B virus; RNA, antisense; mice, transgenic 【摘要】 目的： 探讨针对乙型肝炎病毒(HBVX区反义ＲＮＡ和P区反义ＲＮＡ对乙型肝炎转基因小鼠HBV复制和表达的影响. 方法： 构建表达HBV X区反义RNA和P区反义RNA的重组载体质粒,与脂质体混合，经尾静脉注入小鼠体内，用荧光聚合酶链反应定量法检测血清HBV DNA含量，用NestedPCR法检测血清HBV DNA转阴率. 结果： PLXSNasX小鼠血清HBV DNA的复制于给药后2 d被抑制，至给药后8周抑制依然存在；PLXSNasP小鼠血清HBV DNA的复制于给药后4 wk被抑制，至给药后8周抑制处于上升趋势. 与给药前相比，小鼠血清HBV DNA的复制，在PLXSNasX组和PLXSNasP组分别于2和8 wk达到抑制高峰，抑制率均为58%； 8周后小鼠血清HBV DNA转阴率PLXSNasX组和PLXSNasP组分别为25.0%(2/8, 12.5%(1/8. 结论： HBV Ｘ区反义RNA与HBV P区反义RNA对乙型肝炎病毒转基因鼠抗HBV作用效率无显著差异，但Ｘ区反义RNA的起效时间明显早于P区反义RNA. 【关键词】
